Abstract Dr. Gregerson
Isolation of Xenopus TCS Binding Protein
Robert Gregerson, PhD
Abstract
The translational control sequence (TCS) is a recently-discovered element found in the 3’ untranslated region of the Mos and Wee1 mRNAs of Xenopus laevis. This sequence is a cis-acting element that contributes to the complex pattern of translational control found in these two maternally-derived oocyte mRNAs. It is hypothesized that the TCS functions through an interaction with a sequence-specific RNA binding protein [TCS-binding protein (TCSBP)]. The goal of this project is to obtain cDNA clones corresponding to the Xenopus TCSBP using yeast three-hybrid technology. Analysis of the DNA sequence will reveal whether this protein(s) is homologous to known RNA-binding proteins, or represents a new category of regulator. This information may be relevant not only to meiotic cell cycle progression and oocyte maturation, but to other systems in which complex translational control is an important mechanism for providing temporal regulation of protein expression.
Updated 10/31/2005
The Arkansas INBRE is Supported by a grant from the
National Institutes of Health
and the
National Center
for Research Resources (P20 RR-16460).
Please contact Caroline Miller Robinson regarding questions or comments about this site or our program. For more information about the University of Arkansas for Medical Sciences visit http://www.uams.edu.
