Abstract Dr. Walker
Evaluation of Thiazolidines for Ability to Prevent Oxidative Damage to Membrane Lipids
Richard B. Walker, PhD
University of Arkansas
Abstract
Five thiazolidines which we have synthesized in our laboratory will be evaluated for ability to prevent oxidative damage to membrane lipids using three models. Liposomes will be prepared to simulate membrane lipids. In the first and second models, liposomes will be exposed to hydroxyl radical generated from 2,2-azobis-(amidinopropane) hydrochloride (AAPH) and will be assayed for oxidative damage using the TBARS and N-methyl-2-phenylindole tests. In the third model, liposomes will be exposed to molecular oxygen and oxygen uptake and conjugated diene formation will be monitored. This model allows kinetic parameters to be calculated.
Updated 10/31/2005
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