Abstract Dr. D. Pierce
Ethanol Induced Purkinje Neuron Death
The present study was designed to evaluate the mechanism by which a single ethanol dose delivered intragastrically on postnatal day 4 to rat pups triggers apoptosis of Purkinje cell neurons in the cerebellum. The hypothesis being tested is that ethanol exposure results in the death of the Purkinje neurons by inducing intracellular oxidative stress and interrupting intracellular signaling resulting in neurotrophic factor withdrawal. Data will be collected at various time points subsequent to single ethanol dose to determine the timing of events which shift intracellular signaling away from survival and towards cell death by apoptosis. Immunohistochemical techniques will be utilized to identify Purkinje neurons experiencing oxidative stress and/or alterations in intracellular signaling subsequent to ethanol exposure.
Updated 10/31/2005
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