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The DNA Damage and Toxicology Core provides
expertise, equipment and facilities to perform DNA damage and
toxicology studies related to toxic or hypoxic tissue/cell
injury in drug development, diseases or aging. In addition to
offering standard cytotoxicity assays, the core can measure
oxidative damage and quantify levels of apoptosis and necrosis
in cells and tissues by using quantitative cytochemistry,
immunocytochemistry techniques, and 3-D imaging. Services Provided:
Technology Description
The mechanisms of toxic tissue injury and cell death underlie numerous
human diseases as well as the effects of pharmaceutical drugs and
environmental toxins. These mechanisms are complex and vary with tissue
structure, vascularization, spectrum of cell death proteins, nature of
damaging agent, the strength, length and frequency of the exposure to
the injury, and many other factors. Accordingly, cell death can take
forms of apoptosis, necrosis, autophagy, mitotic catastrophe, as well as
some intermediate or more specific forms of cell death including
aponecrosis, oncosis, anoikis or abortosis. To approach this complexity,
the best strategy for assessment of toxic tissue injury includes the use
of: a) common methods that occur in all injuries independent of causes,
mechanisms or the origin of tissue; b) methods that are quantitative;
and c) approaches that allow, if necessary, combining observations with
other methods to determine the mechanism of cell injury and death.
Contact information: DNA Damage & Toxicology Core Alexei Basnakian, M.D.,
Ph.D., Director
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