http://www.acrc.uams.edu/cancer_research/proteomics.asp

 Contact Information  |  Links  |  Proteomics UAF

The Arkansas Cancer Research Center and Arkansas INBRE support the Proteomics Core Facility, located in the UAMS Biomedical Research Center Building 1. This facility also serves as part of the Biotechnology Core for Arkansas INBRE. It provides the service of protein characterization by mass spectrometry and 2-D gel analysis.

The laboratory is equipped with a Micromass Q-TOF Micro mass spectrometer, a Thermo Finnigan MALDI-LTQ mass spectrometer, a ProPic imaging and spot-picking robot from Genomic Solutions, a ProGest in-gel enzymatic digestion robot from Genomic Solutions, and a Bio-Rad 2D gel electrophoresis system. The Q-TOF Micro is interfaced with a Micromass CapLC system equipped with a nanoflow electrospray interface. These instruments are capable of sequencing and identifying purified proteins or complex mixtures. Both MS and MS/MS analyses can be performed.

An IBM dual processor server allows for database searching of mass spectrometry data. The ProPic instrument, from Genomic Solutions, has interchangeable visible and ultraviolet light sources to allow for visualization of gels stained with various types of dyes. Using the Investigator software, spots from 2D gels or bands from 1D gels can be selected for excision into 96-well plates. In addition, the Investigator software can be used to create 2D gel overlays, allowing for determination of variable protein levels between two or more samples. The ProGest instrument, from Genomic Solutions, is a programmable robot that can simultaneously perform in-gel enzymatic digestions on up to 96 samples.

High Throughput Protein Analysis

For more information, contact:

Proteomics Core Leader

Dr. Kevin D. Raney
Associate Professor
University of Arkansas for Medical Sciences
Dept. of Biochemistry & Molecular Biology
(501) 686-5244

UAMS Mass Spectrometry:

Dr. Samuel G. Mackintosh
Instructor
University of Arkansas for Medical Sciences
Dept. of Biochemistry & Molecular Biology
(501) 526-5978
 

2-D gels:
Chris Warthen
Research Assistant

University of Arkansas for Medical Sciences

Dept. of Biochemistry & Molecular Biology

(501) 526-5978

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UAF Center for Protein Structure and Function

The Arkansas BRIN is also supported by facilities at the University of Arkansas, Fayetteville through the Center for Protein Structure and Function.  Facilities include x-ray crystallography, high-field NMR, and mass spectrometry.   For more information about the Center for Protein Structure and Function, visit out the following web site: http://www.uark.edu/depts/cheminfo/uarkchem/protein/index.html

For more information, contact:

Frank Millett, PhD

Director of CPSF,
Department of Chemistry and Biochemistry
University of Arkansas
Fayetteville, Arkansas 72701
Phone: 479-575-4999

Statewide Mass Spectrometry Facility

University of Arkansas, Fayetteville - http://www.uark.edu/depts/anylchem/Spec/index.html

For more information, contact:

Jackson O. Lay, PhD
Director - Arkansas Statewide Mass Spectrometry Facility
Phone: (479) 575-2018
 

Links  

Journal of Proteome Research - ACS proteomics journal

Molecular & Cellular Proteomics - ASBMB proteomics journal